andor.com© Andor Technology plc 2013iXon UltraHardware GuideVersion 1.1 rev Jan 2013
Version 1.1 rev Jan 2013Page 10iXon UltraSection TitleAPPENDIX 98A.1 GLOSSARY 98A.1.1 READOUT SEQUENCE OF AN EMCCD 98A.1.2 ACCUMULATION 99A.1.3 AC
Version 1.1 rev Jan 2013Page 100iXon UltraAppendixA.1.9 - Detection LimitThe Detection Limit is a measure of the smallest signal that can be detected
Version 1.1 rev Jan 2013Page 101iXon UltraAppendixA.1.12 - NOISEA.1.12.1 - Pixel NoiseThe Pixel Noise is the variation in the pixel’s charge level whe
Version 1.1 rev Jan 2013Page 102iXon UltraAppendixA.1.12.1.3 - Calculation of Total Pixel NoiseThe total pixel noise is not simply the sum of the thre
Version 1.1 rev Jan 2013Page 103iXon UltraAppendixA.1.16 - Scans (Keep Clean and Acquired)The EMCCD is continually being “scanned” to prevent its beco
Version 1.1 rev Jan 2013Page 104iXon UltraAppendixB - MECHANICAL DIMENSIONS
Version 1.1 rev Jan 2013Page 105iXon UltraAppendixC - DECLARATION OF CONFORMITY
Version 1.1 rev Jan 2013Page 106iXon UltraAppendix
Version 1.1 rev Jan 2013Page 107iXon UltraAppendixD - HARDWARE AND SOFTWARE WARRANTY SERVICED.1 - SERVICE DESCRIPTIOND.1.1 The Andor Repair service p
Version 1.1 rev Jan 2013Page 108iXon UltraAppendixD.3 - Hardware RemediationD.3.1 If the issue cannot be resolved remotely and a fault has been diagn
Version 1.1 rev Jan 2013Page 109iXon UltraAppendixD.4 - Software RemediationD.4.1 During Warranty Customers have access to the Service Desk at www.an
Version 1.1 rev Jan 2013Page 11iXon UltraSection TitleA.1.16 SCANS (KEEP CLEAN AND ACQUIRED) 103A.1.17 SHIFT REGISTER 103A.1.18 SIGNAL TO NOISE R
Version 1.1 rev Jan 2013Page 12iXon UltraSAFETY AND WARNINGS INFORMATIONSafety and Warnings Information PLEASE READ THIS INFORMATION FIRST1.
Version 1.1 rev Jan 2013Page 13iXon Ultra About the Andor iXon UltraSAFETY AND WARNINGS SYMBOLSThe following are explanations of the symb
Version 1.1 rev Jan 2013Page 14iXon UltraSECTION 1 - INTRODUCTION TO IXON ULTRA HARDWARE Introduction to iXon Ultra HardwareThank you for choosing the
Version 1.1 rev Jan 2013Page 15iXon UltraIntroduction to iXon Ultra Hardware 1.2 - DISCLAIMERThe information contained herein is provided "as is&
Version 1.1 rev Jan 2013Page 16iXon UltraIntroduction to iXon Ultra Hardware 1.4 - COMPONENTSThe Andor iXon Ultra system comprises the following main
Version 1.1 rev Jan 2013Page 17iXon UltraIntroduction to iXon Ultra Hardware 1.4.1 - Camera descriptionThe iXon Ultra camera is shown below. The camer
Version 1.1 rev Jan 2013Page 18iXon Ultra1.4.2 - Camera Power Supply UnitThe iXon Ultra system is designed to be powered from an SW4189 external PSU (
Version 1.1 rev Jan 2013Page 19iXon UltraIntroduction to iXon Ultra Hardware 1.5 - SPECIFICATIONSPARAMETER SPECIFICATIONPower supply ratings 100 - 240
Version 1.1 rev Jan 2013Page 2iXon UltraTable of ContentsSAFETY AND WARNINGS INFORMATION 12SAFETY AND WARNING SYMBOLS 13MANUAL HANDLING 13SHIPPING
Version 1.1 rev Jan 2013Page 20iXon UltraIntroduction to iXon Ultra Hardware 1.7 - SAFETY PRECAUTIONS AND MAINTENANCE1.7.1 - Care of the cameraWARNING
Version 1.1 rev Jan 2013Page 21iXon UltraIntroduction to iXon Ultra Hardware 1.7.2 - Regular checksThe state of the product should be checked regularl
Version 1.1 rev Jan 2013Page 22iXon Ultra Introduction to iXon Ultra Hardware 1.7.6 - Working with electronicsThe computer equipment that is to be use
Version 1.1 rev Jan 2013Page 23iXon UltraIntroduction to iXon Ultra Hardware 1.7.8 - Dew Point graphThe relationship between Relative Humidity and Dew
Version 1.1 rev Jan 2013Page 24iXon UltraIntroduction to iXon Ultra Hardware 1.7.9 - EM Gain ageingIt has been observed that some EMCCD sensors, more
Version 1.1 rev Jan 2013Page 25iXon UltraIntroduction to iXon Ultra Hardware 1.7.10 - Minimizing particulate contaminationIt is important that particu
Version 1.1 rev Jan 2013Page 26iXon UltraInstallationSECTION 2: INSTALLATION 2.1 - INSTALLING THE HARDWARE2.1.1- PC requirementsInstall the camera Sof
Version 1.1 rev Jan 2013Page 27iXon UltraInstallation 2.2 - INSTALLING ANDOR SOLIS SOFTWARE - WINDOWS O/S(XP/VISTA/SEVEN)1. Terminat
Version 1.1 rev Jan 2013Page 28iXon Ultra5. Select iXon Ultra, then click Next> and the following dialog box appears:6. Click Next > (alternativ
Version 1.1 rev Jan 2013Page 29iXon UltraInstallation 8. Click Install to continue with the installation (or click <Back, if you want to review / c
Version 1.1 rev Jan 2013Page 3iXon UltraTable of Contents 1.5 SPECIFICATIONS 191.6 ACCESSORIES 191.7 SAFETY PRECAUTIONS AND MAINTENANCE 201.7.1
Version 1.1 rev Jan 2013Page 30iXon UltraInstallation 2.3 - NEW HARDWARE WIZARDWhen the rst iXon Ultra camera is connected to a PC for the rst time,
Version 1.1 rev Jan 2013Page 31iXon UltraInstallation 4. The installation wizard runs, e.g.: 5. When hardware installation is complete, the followin
Version 1.1 rev Jan 2013Page 32iXon UltraInstallation 2.4 - CONNECTORSThe Power connection is for the Power Supply Unit (PSU) described in Section 1.4
Version 1.1 rev Jan 2013Page 33iXon UltraInstallation 2.5 - WATER PIPE CONNECTORSTwo barbed coolant hose inserts are supplied as standard with the iXo
Version 1.1 rev Jan 2013Page 34iXon UltraInstallation2.6 - MOUNTING POSTS• Mountingpostscanbettedonallfoursidesofthecamera.Thesecanbe
Version 1.1 rev Jan 2013Page 35iXon Ultra2.7 - COOLINGCooling of a CCD or EMCCD sensor allows efcient reduction of dark current and unwanted silicon
Version 1.1 rev Jan 2013Page 36iXon UltraInstallation2.8 - START-UP DIALOGOn start-up of the Solis software a dialog will appear, offering a selection
Version 1.1 rev Jan 2013Page 37iXon Ultra, Features and FunctionalitySECTION 3 - FEATURES AND FUNCTIONALITY 3.1 - EMCCD OPERATION3.1.1 - Structure of
Version 1.1 rev Jan 2013Page 38iXon UltraFeatures and FunctionalityDuring an acquisition using a conventional Frame Transfer CCD (FT CCD), the image a
Version 1.1 rev Jan 2013Page 39iXon Ultra, Features and Functionality3.1.2 - EM Gain & Read Noise As explained in Section 3.1.1, EMCCD sensors all
Version 1.1 rev Jan 2013Page 4iXon UltraTable of Contents SECTION 2: INSTALLATION 262.1 INSTALLING THE HARDWARE 262.1.1 PC requirements 262.2 IN
Version 1.1 rev Jan 2013Page 40iXon UltraFeatures & Functionality3.1.3 - EM Gain ON vs EM Gain OFFSignal to Noise (S/N) plots derived from the spe
Version 1.1 rev Jan 2013Page 41iXon Ultra, Features and FunctionalityS/N plots derived from the specications of the back-illuminated iXon Ultra EMCCD
Version 1.1 rev Jan 2013Page 42iXon Ultra, Features and Functionality3.1.4 - Multiplicative Noise Factor and Photon CountingIt is impossible to determ
Version 1.1 rev Jan 2013Page 43iXon UltraFigure 11: Signal intensity proleWithin the framework of less than 1 electron falling on a pixel in a single
Version 1.1 rev Jan 2013Page 44iXon UltraFeatures & Functionality3.1.5 - EM Gain dependence and stabilityEM Gain is a function of the EM voltage a
Version 1.1 rev Jan 2013Page 45iXon Ultra, Features and Functionality3.1.6 - RealGainTM: Real and Linear gainAndor has successfully converted the rela
Version 1.1 rev Jan 2013Page 46iXon Ultra, Features and Functionality3.1.7 - EM Gain Ageing: What causes it and how is it countered?As already noted i
Version 1.1 rev Jan 2013Page 47iXon Ultra, Features and Functionality3.1.8 - Gain and signal restrictions Part of the measures taken to prevent premat
Version 1.1 rev Jan 2013Page 48iXon Ultra, Features and Functionality3.2 - COOLINGThe EMCCD sensor is cooled using a Thermoelectric (TE) cooler. TE co
Version 1.1 rev Jan 2013Page 49iXon Ultra, Features and FunctionalityNOTE: The OptAcquire “Fastest Frame Rate” setting in Solis uses the fastest verti
Version 1.1 rev Jan 2013Page 5iXon UltraTable of Contents SECTION 3: FEATURES AND FUNCTIONALITY 373.1 EMCCD OPERATION 373.1.1 Structure of an EMCC
Version 1.1 rev Jan 2013Page 50iXon Ultra, Features and Functionality3.3.1 - Sensor Pre-amp optionsAn EMCCD sensor can have a much larger dynamic rang
Version 1.1 rev Jan 2013Page 51iXon Ultra, Features and FunctionalityAndor recommends using the default highest value pre-amp setting (e.g. Gain3 sett
Version 1.1 rev Jan 2013Page 52iXon Ultra, Features and Functionality3.3.2 - Variable Horizontal Readout RateThe Horizontal Readout Rate denes the ra
Version 1.1 rev Jan 2013Page 53iXon Ultra, Features and Functionality3.3.4 - Output amplier selection The iXon Ultra camera incorporates dual output
Version 1.1 rev Jan 2013Page 54iXon Ultra, Features and Functionality3.3.5 - Baseline Optimization3.3.5.1 - Baseline ClampWhen acquiring data, small c
Version 1.1 rev Jan 2013Page 55iXon Ultra, Features and Functionality3.3.6 - Binning and Sub Image optionsBinning is a process that allows charge from
Version 1.1 rev Jan 2013Page 56iXon Ultra, Features and Functionality Figure 17: Vertical and Horizontal binning of two rowsStep 1 Charge is built up
Version 1.1 rev Jan 2013Page 57iXon Ultra, Features and Functionality3.4 - ACQUISITION OPTIONS3.4.1 - Capture Sequence in Frame Transfer (FT) ModeA nu
Version 1.1 rev Jan 2013Page 58iXon Ultra, Features and Functionality3.4.1.1 - Points to consider when using FT Mode• Inthismode,therearenoKeep
Version 1.1 rev Jan 2013Page 59iXon Ultra, Features and Functionality3.4.2 - Capture Sequence in Non-Frame Transfer Mode (NFT) with an FT EMCCDIt is a
Version 1.1 rev Jan 2013Page 6iXon UltraTable of Contents 3.3.6 Binning and Sub Image options 553.4 ACQUISITION OPTIONS 573.4.1 Capture Sequence
Version 1.1 rev Jan 2013Page 60iXon Ultra, Features and Functionality3.4.2.1 - Points to note about using an FT EMCCD as a standard EMCCD• Theexposu
Version 1.1 rev Jan 2013Page 61iXon Ultra, Features and Functionality3.4.3 - Capture Sequence for Fast Kinetics (FK) with an FT EMCCD Fast Kinetics (F
Version 1.1 rev Jan 2013Page 62iXon Ultra, Features and Functionality3.4.4 - Keep Clean CyclesiXon Ultra cameras have a range of different Keep Clean
Version 1.1 rev Jan 2013Page 63iXon Ultra, Features and FunctionalityThe second type of Keep Clean Cycle is executed between individual scans in a kin
Version 1.1 rev Jan 2013Page 64iXon Ultra, Features and FunctionalityThe third Keep Clean is the External Keep Clean Cycle. This cycle uses a differen
Version 1.1 rev Jan 2013Page 65iXon Ultra, Features and Functionality3.5 - TRIGGERING OPTIONSThe iXon Ultra camera has several different triggering mo
Version 1.1 rev Jan 2013Page 66iXon Ultra, Features and Functionality3.5.1 - Triggering options in Frame Transfer (FT) mode3.5.1.1 - Internal Triggeri
Version 1.1 rev Jan 2013Page 67iXon Ultra, Features and Functionality3.5.1.2 - External Triggering (FT)When the camera is idle, it runs the Idle Keep
Version 1.1 rev Jan 2013Page 68iXon Ultra, Features and FunctionalitySince all iXon Ultra cameras have iCam technology, the rising edge of the externa
Version 1.1 rev Jan 2013Page 69iXon Ultra, Features and Functionality3.5.1.3 - External Exposure (FT)This mode is distinct from the triggering modes d
Version 1.1 rev Jan 2013Page 7iXon UltraTable of Contents 3.5.3.2 External (FK) 753.5.3.3 External Start (FK) 763.6 SHUTTERING 773.7 COUNT CONVE
Version 1.1 rev Jan 2013Page 70iXon Ultra, Features and Functionality3.5.2 - Triggering options in Non-Frame Transfer (NFT) mode3.5.2.1 - Internal (NF
Version 1.1 rev Jan 2013Page 71iXon Ultra, Features and Functionality3.5.2.2 - External & Fast External (NFT)In External Trigger modes, once an ac
Version 1.1 rev Jan 2013Page 72iXon Ultra, Features and Functionality3.5.2.3 - External Exposure (NFT)This mode is distinct from the triggering modes
Version 1.1 rev Jan 2013Page 73iXon Ultra, Features and Functionality3.5.2.4 - Software trigger (NFT) This mode is particularly useful when the user n
Version 1.1 rev Jan 2013Page 74iXon Ultra, Features and Functionality3.5.3 - Trigger options in Fast Kinetics (FK) mode3.5.3.1 - Internal (FK)As Fast
Version 1.1 rev Jan 2013Page 75iXon Ultra, Features and Functionality3.5.3.2 - External (FK)In External Trigger mode, a trigger pulse is required to s
Version 1.1 rev Jan 2013Page 76iXon Ultra, Features and Functionality3.5.3.3 - External Start (FK)External Start trigger mode is a combination of Exte
Version 1.1 rev Jan 2013Page 77iXon Ultra, Features and Functionality3.6 - SHUTTERINGThe iXon Ultra camera is supplied with a built in bi-stable shutt
Version 1.1 rev Jan 2013Page 78iXon Ultra, Features and Functionality3.7 - COUNT CONVERTOne of the distinctive features of the iXon Ultra is the capab
Version 1.1 rev Jan 2013Page 79iXon Ultra, Features and Functionality3.8 - OPTACQUIREOptAcquire is a unique control interface, whereby a user can conv
Version 1.1 rev Jan 2013Page 8iXon UltraTable of Contents SECTION 4: HARDWARE 874.1 EMCCD TECHNOLOGY 874.1.1 What is an Electron Multiplying CCD?
Version 1.1 rev Jan 2013Page 80iXon Ultra, Features and Functionality3.8.1 - OptAcquire modesPre-dened OptAcquire modes include:Sensitivity and Speed
Version 1.1 rev Jan 2013Page 81iXon Ultra, Features and Functionality3.9 - PUSHING FRAME RATES WITH CROPPED SENSOR MODEThe iXon Ultra offers Cropped S
Version 1.1 rev Jan 2013Page 82iXon Ultra, Features and Functionality3.9.1 - Cropped Sensor Mode Frame RatesIn biological imaging, Cropped Sensor Mode
Version 1.1 rev Jan 2013Page 83iXon Ultra, Features and Functionality3.10 - ADVANCED PHOTON COUNTING IN EMCCDSPhoton Counting in EMCCDs is a way to ov
Version 1.1 rev Jan 2013Page 84iXon Ultra, Features and FunctionalityTo successfully photon count with EMCCDs, there has to be a signicantly higher p
Version 1.1 rev Jan 2013Page 85iXon Ultra, Features and Functionality3.10.1 - Photon Counting by Post-ProcessingAs a post-processing analysis, the use
Version 1.1 rev Jan 2013Page 86iXon Ultra, Features and Functionality3.11 - SPURIOUS NOISE FILTERIt can be desirable to optionally lter spurious EM-a
Version 1.1 rev Jan 2013Page 87iXon UltraSECTION 4: HARDWAREHardware4.1 - EMCCD TECHNOLOGY4.1.1 - What is an Electron Multiplying CCD?Current trends i
Version 1.1 rev Jan 2013Page 88HardwareiXon Ultra4.1.4 - What applications are EMCCDs suitable for?EMCCD based detectors have been designed for the mo
Version 1.1 rev Jan 2013Page 89iXon UltraHardware4.2 - EMCCD SENSORThe EMCCD sensor is the core enabling technology of the system. Everything else in
Version 1.1 rev Jan 2013Page 9iXon UltraTable of ContentsSECTION 5: TROUBLESHOOTING 955.1 UNIT DOES NOT SWITCH ON 955.2 SUPPORT DEVICE NOT RECOGNI
Version 1.1 rev Jan 2013Page 90iXon UltraHardware4.3 - VACUUM HOUSINGUnless protected, cooled CCD sensors will condense moisture, hydrocarbons and oth
Version 1.1 rev Jan 2013Page 91iXon UltraHardware4.3.1 - Thermoelectric coolerThe iXon Ultra range makes use of a four-stage Peltier cooling assembly,
Version 1.1 rev Jan 2013Page 92iXon UltraHardware4.4 – USB 2.0 INTERFACEUSB 2.0 is a convenient interface standard for use with a Scientic camera, as
Version 1.1 rev Jan 2013iXon UltraPage 93Hardware4.5 - OUTGASSINGOutgassing is the release of a gas trapped in a material. It is a problem encountered
Version 1.1 rev Jan 2013Page 94iXon UltraHardware4.7 - SIGNAL DIAGRAMSiXon Ultra Input & Output Timing HardwareFigure 42: External TriggerExternal
Version 1.1 rev Jan 2013Page 95iXon UltraTroubleshooting5.1 - UNIT DOES NOT SWITCH ON• Checkpowercordispluggedinandconnectedcorrectlytomain
Version 1.1 rev Jan 2013Page 96iXon UltraTroubleshooting5.4 - CAMERA HIGH FIFO FILL ALARMOn some systems, it has been observed that a camera will stop
Version 1.1 rev Jan 2013Page 97iXon UltraTroubleshootingTo map each port, use a High Bandwidth device such as a modern USB memory stick. High bandwidt
Version 1.1 rev Jan 2013Page 98iXon UltraAppendixA.1 - GLOSSARYThis glossary provides an overview of the concepts and terminology used in Andor’s EMCC
Version 1.1 rev Jan 2013Page 99iXon UltraAppendixA.1.2 - AccumulationAccumulation is the process by which data that have been acquired from a number o
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